FoxP3/CD4 vs FoxP3/CD25

Ramunas J. ramujana at yahoo.com
Fri Oct 21 15:23:06 EST 2005


Dear clinical FCM users,
i'd like to address several questions to your global
audience concerning Treg(FoxP3) staining:
1. is there the most preferable combination for
choosing between bivariate FCM profile of human
FoxP3/CD4 and FoxP3/CD25 cells? Which combination is
better? I mean, why should i use both CD4 and CD25 if
it's possible to choose only one of them (this is
clinical setting, not scientific - it's a matter of
cost efficiency);
2.what clone is preferable to use? PCH101?

thank you in advance!



Ramunas Janavicius
Institute of Immunology,
Vilnius University
Vilnius, Lithuania



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