SP on Aria with violet laser

Jodene K Moore drjmoore at temple.edu
Wed Nov 30 13:43:56 EST 2005

Hello-Has anyone successfully attempted stem cell isolation 
by sorting SP on the Aria, having excited the Hoechst with 
the violet laser? If so, can you tell me your filter config 
and give me any suggestions on optimizing the setup under 
these suboptimal conditions? Thanks for your help!
Jodi Moore, PhD
Flow Cytometry Facility Manager
Temple University, School of Medicine
Fels Institute of Cancer Research and Molecular Biology
Room 315, AHP
3307 North Broad Street
Philadelphia, PA 19140


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