SP Cells

Berend Hooibrink B.Hooibrink at amc.uva.nl
Tue Nov 29 03:57:50 EST 2005

Hi Leonie,

Have a look in Current Protocols in Cytometry section 9.18. Here you
will find the information you are looking for (and a lot more).

Berend Hooibrink Ing.
FACS facility
Academic Medical Center
Dept. of Cell Biology & Histology
Room L3-115, L3-139
Meibergdreef 9
1105 AZ Amsterdam
The Netherlands
Tel: + 31-20-5666804, +31-20-5664676
Email:	b.hooibrink at amc.uva.nl

-----Original Message-----
From: Leonie Gaudry [mailto:L.gaudry at unsw.EDU.AU] 
Sent: maandag 28 november 2005 6:43
To: cyto-inbox
Subject: SP Cells

I am having a bit of trouble setting up the Voltages and Compensation
SP Cells.
I am acquiring the cells on a BD FACSVantage with DiVa option in Digital
How critical are the filters,I have a Hoechst 33342 450/20 for the blue
a 660/20 for the red but I only have a 525DCSP for the splitter. Are
okay or not specific enough?

I also have a problem in the two papers I am referring too. One paper
states that the detectors should be in Linear mode while a second paper
in Log. Up till now I have been running in Log so which is correct and I
would be very interested to hear anyones experiences.
Thank you for your help.
Best Wishes

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