DsRed labeled cell sorting

zhao wenping zhao at med.yokohama-cu.ac.jp
Wed Nov 16 07:12:02 EST 2005


Dear flowers,
I'm using MoFlo cell sorter to sort DsRed labeled cells, I set PE channel 
(bandpass filter 580/30) for DsRed and PE-Cy5 channel (670/30) for 7AAD, it 
should not be but it turned out that the spillover from DsRed to 7AAD is 
above 40%, is there any problem with channel setting?
Thanks in advance.

Wenping Zhao
Department of Regenerative Medicine
Yokohama City University School of Medicine
3-9, FuKu-ura, Kanazawa Ku, Yokohama
236-0004, Japan
Tel/Fax:  045-787-8963(Lab)
E-mail: zhao at med.yokohama-cu.ac.jp 
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