CD107a-tetramer double staining

McCloskey, Thomas thomasm at nshs.EDU
Fri Nov 11 15:09:53 EST 2005


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From: Sandra Tuyaerts [mailto:Sandra.Tuyaerts at vub.ac.be] 
 The T cells restimulated with relevant peptide-pulsed T2 cells show a nice
CD107a positivity, but unfortunately these cells don't stain positive with
the tetramers anymore. 
 What could have happened during this co-culture that the cells become
tetramer negative? Can anybody give us a hint? 
H i Sandra,
    If I understand your system correctly, perhaps the peptide from your T2
cells is binding the receptor on the T cells.	 So you may not be able to
tag the tetramer onto its receptor because the receptor is already loaded
with peptide or the peptide has cauused internalization of the receptor.  
regards,
Tom
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Thomas W. Mc Closkey, Ph. D. 
Scientist in Charge, Flow Cytometry 
The Institute for Medical Research, North Shore University Hospital 
Assistant Professor of Pediatrics, New York University School of Medicine 
Biomedical Research Center, 350 Community Drive 
Manhasset, Long Island, New York 11030 
ph:  516-562-4844 [office], 516-562-1084 [lab] 
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