CD107a-tetramer double staining

McCloskey, Thomas thomasm at nshs.EDU
Fri Nov 11 15:09:53 EST 2005


From: Sandra Tuyaerts [mailto:Sandra.Tuyaerts at] 
 The T cells restimulated with relevant peptide-pulsed T2 cells show a nice
CD107a positivity, but unfortunately these cells don't stain positive with
the tetramers anymore. 
 What could have happened during this co-culture that the cells become
tetramer negative? Can anybody give us a hint? 
H i Sandra,
    If I understand your system correctly, perhaps the peptide from your T2
cells is binding the receptor on the T cells.	 So you may not be able to
tag the tetramer onto its receptor because the receptor is already loaded
with peptide or the peptide has cauused internalization of the receptor.  
Thomas W. Mc Closkey, Ph. D. 
Scientist in Charge, Flow Cytometry 
The Institute for Medical Research, North Shore University Hospital 
Assistant Professor of Pediatrics, New York University School of Medicine 
Biomedical Research Center, 350 Community Drive 
Manhasset, Long Island, New York 11030 
ph:  516-562-4844 [office], 516-562-1084 [lab] 


The information contained in this electronic e-mail transmission
and any attachments are intended only for the use of the individual
or entity to whom or to which it is addressed, and may contain
information that is privileged, confidential and exempt from
disclosure under applicable law. If the reader of this communication
is not the intended recipient, or the employee or agent responsible
for delivering this communication to the intended recipient, you
are hereby notified that any dissemination, distribution, copying
or disclosure of this communication and any attachment is strictly
prohibited. If you have received this transmission in error, please
notify the sender immediately by telephone and electronic mail,
and delete the original communication and any attachment from any
computer, server or other electronic recording or storage device
or medium. Receipt by anyone other than the intended recipient is
not a waiver of any attorney-client, physician-patient or other
privilege. Thank you.

-------------- next part --------------
HTML attachment scrubbed and removed

More information about the Cytometry mailing list