bacteria by flow
Corrado.Cilio at med.lu.se
Thu Nov 10 17:07:23 EST 2005
For the first time we are thinking to run bacteria in our FACScalibur.
Is there someone that can give me some good advices?
Basic questions like: 1. it is ok to resuspend in normal FACS buffer (FCS, azide) 2.
which would be the best instrument settings to get started (mostly FSC and SSC). We want
to look at cultured e-coli/eGFP and fecal samples which should contain the same bugs. 3.
cell concentrations? 4. Special cleaning advices?
Thanks in advance
Corrado M. Cilio, M.D., Ph.D.
Cellular Autoimmunity Unit
Dept. of Clinical Sciences/Paediatrics
Malmo University Hospital
205 02 Malmo, Sweden
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