bacteria by flow

Corrado Cilio Corrado.Cilio at
Thu Nov 10 17:07:23 EST 2005


For the first time we are thinking to run bacteria in our FACScalibur. 
Is there someone that can give me some good advices? 
Basic questions like: 1. it is ok to resuspend in normal FACS buffer (FCS, azide) 2.
which would be the best instrument settings to get started (mostly FSC and SSC). We want
to look at cultured e-coli/eGFP and fecal samples which should contain the same bugs. 3.
cell concentrations? 4. Special cleaning advices?

Thanks in advance


Corrado M. Cilio, M.D., Ph.D. 
Assistant Professor 
Cellular Autoimmunity Unit 
Dept. of Clinical Sciences/Paediatrics 
Malmo University Hospital 
Lund University 
205 02 Malmo, Sweden 

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