A simple question

jtigges@caregroup.harvard.edu jtigges at caregroup.harvard.edu
Tue Nov 8 14:47:22 EST 2005


Hello All,
I was wondering if anyone had any insight on staining plant nuclei with PI.  We have a researcher who is staining plant nuclei using Otto I and then Otto II buffer with seperate PI(2 step procedure).  We have been having problems establishing the DNA content model.
Thanks in Advance,
 
John Tigges 
 
Core Facility Manager
Flow Cytometry
Beth Israel Deaconess Medical Center
330 Brookline Avenue
Boston, MA 02215
(617) 667-4901
(617) 975-5536

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