[ Platelet activation question ]

Masa Sasagawa masas at bastyr.edu
Tue Nov 8 15:56:06 EST 2005


How about inflammatory mediators to activate platelets, such as
histamine, thrombin, or phorbol ester. The gene expression of p-selectin
is best measured at 10 min (temp dependent). P-selection can be measured
in flow (CD62P) , but be careful when optimizing assay because there
seems to be a secondary surge of P-selectin after the initial raise
declines.
 
Best,   
Masa Sasagawa, ND
Naturopathic Physician - Washington
Affiliate Assistant Professor - University of Washington
  School of Nursing, Dept of Biobehavioral Nursing and Health Systems
Postdoctoral Research Fellow - Bastyr University
14500 Juanita Dr NE, #472
Kenmore, WA 98028
Phone: 425-602-3178
Fax: 425-602-3079
masas at bastyr.edu
 

________________________________

From: Nebe-Von-Caron, G [mailto:g.nebe-von-caron at unipath.com] 
Sent: Tuesday, November 08, 2005 7:21 AM
To: cyto-inbox
Subject: RE: [ Platelet activation question ]


you can measure platelet activation by their binding to monocytes which
is a marker for heart risk, but cell cell aggregation should not be
confused with platelet activation as it can occur independently
particularly when washing and centrifugation steps are part of the
sample preparation. You can measure that separately by a range of
surface markers shown on the platelet, but you have to be aware of the
problems of sampling and sample storage to prevent the platelets going
off.
 
When measuring platelet - leukocyte aggregation one has to distinguish
true binding events from coincident events. In case of heart risk the
binding to the monocots should be specific, e.g be higher in percentage
than for the other cells, easies seen in a log side scatter versus the
respective CD markers. Not having researched the field in detail but
looked ad adhesion molecules some time ago I would just want to make
people aware that adhesion molecule expression is a question of
anticoagulant and time and temperature, and so should be cell adhesion.
There are a lot of confusing articles out about the phenomenon of cell
to platelet aggregation that lack systematic controls. 
 
Regards
 
Gerhard

	-----Original Message-----
	From: KurtzJ at usa.redcross.org [mailto:KurtzJ at usa.redcross.org] 
	Sent: 07 November 2005 15:17
	To: Cytometry Mailing List
	Subject: [ Platelet activation question ]
	
	
	I would like to know is anyone out there has a procedure to
measusare platelet activation using leukocyte aggregates?  I also have
seen that the measurement 
	of leukocyte aggregates is usually done for invivo studies, is
this also an appropriate measurement for invitro work?	Thanks, any
comments would be 
	appreciated.  Jim Kurtz

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