excitation and emission
d_coder at MSN.com
Mon Nov 7 17:07:31 EST 2005
In fact, it's just right. The 488nm absorption peak of R-PE is a minor
shoulder of excitation (the shoulder is missing B-PE). Phycoerythrin
preferentially absorbs green light, and this a reason why red algae grow at
great depth: Blue light is scattered by particulates and red light is
absorbed by water. PE can absorb green light and pass the energy (via
phycocyanin and allophycocyanin) to chlorophyll for photosynthesis.
David M Coder, Ph.D.
Consultant in Cytometry
Cell/Msg: 206 499 3446
Email: d_coder at msn.com
From: Mark D'Amico [mailto:MDAmico at panaceapharma.com]
Sent: Friday, November 04, 2005 12:16 PM
Subject: excitation and emission
I have a simple, if not outright stupid question.
I have a 532nm laser for excitation. I am using R-PE as my marker. The
excitation peak is at 488nm. Is my laser wavelength too long?
Mark D'Amico, Ph.D.
207 Perry Parkway #2
Gaithersburg, MD 20877
(240) 243-8000 Ext. 206
mdamico at panaceapharma.com
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