excitation and emission

David Coder d_coder at MSN.com
Mon Nov 7 17:07:31 EST 2005


In fact, it's just right. The 488nm absorption peak of R-PE is a minor
shoulder of excitation (the shoulder is missing B-PE). Phycoerythrin
preferentially absorbs green light, and this a reason why red algae grow at
great depth: Blue light is scattered by particulates and red light is
absorbed by water. PE can absorb green light and pass the energy (via
phycocyanin and allophycocyanin) to chlorophyll for photosynthesis.


================

David M Coder, Ph.D.

Consultant in Cytometry

Irvine, CA 

Cell/Msg: 206 499 3446

Email: d_coder at msn.com


  _____  

From: Mark D'Amico [mailto:MDAmico at panaceapharma.com] 
Sent: Friday, November 04, 2005 12:16 PM
To: cyto-inbox
Subject: excitation and emission


Howdy,


I have a simple, if not outright stupid question. 


I have a 532nm laser for excitation. I am using R-PE as my marker. The
excitation peak is at 488nm. Is my laser wavelength too long? 


Mark D'Amico, Ph.D.

Scientist

Panacea Pharmaceuticals

207 Perry Parkway    #2

Gaithersburg, MD   20877

(240) 243-8000 Ext. 206

mdamico at panaceapharma.com


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