PE and PI together?

David Coder d_coder at msn.com
Mon Nov 7 18:24:16 EST 2005


When using PI for identifying dead cells, you may be able to ignore the
compensation of PI into PE because you will gate out any PI-positive cells.
However, you do need to compensate PE into PI such that you don't eliminate
viable PE-bright cells as false non-viable cells. The attached examples are
with 7-AAD which is easier to separate from PE.


Dave


================

David M Coder, Ph.D.

Consultant in Cytometry

Irvine, CA 

Cell/Msg: 206 499 3446

Email: d_coder at msn.com



-----Original Message-----
From: Nancy [mailto:nogden at globelaboratories.com] 
Sent: Thursday, November 03, 2005 5:36 PM
To: Cytometry Mailing List
Subject: PE and PI together?



Dear Flowers:


Can PI be compensated for in a PE-labeled sample that does not exhibit
clearly differentiated positive and negative populations, but a broad peak?
Is measuring cell viability in a separate PI-only sample the norm? 


Thanks again for your help!


Nancy Ogden, M.Sc
Research Associate
Chemokine Therapeutics Corp.
2386 East Mall, Suite 303
Vancouver, B.C., Canada
V6T 1Z3



Tel:  604-822-8848


Fax: 604-822-0212






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