PE and PI together?

Joanne Lannigan joannelannigan at virginia.edu
Mon Nov 7 11:01:04 EST 2005


Nancy:

Why not use 7-AAD instead? It emits in the farther red region and is
certainly compensatable with PE. Another alternative if you have a red laser
is TOPRO-3 or Sytox Green if your green channel is free. If you are gating
out dead cells and you can clearly distinguish them in fluorescent intensity
from your PE signal you can probably get away with PI as a viability marker
without compensation. 

 

Joanne Lannigan, MS

Director, Flow Cytometry Core Facility

University of Virginia

Jordan Hall, Room 7067

P.O. Box 800734

Charlottesville, VA 22908-0734

Office: 434-924-0274

Lab: 434-243-2695

Fax: 434-982-1071

email: joannelannigan at virginia.edu

-----Original Message-----
From: Nancy [mailto:nogden at globelaboratories.com] 
Sent: Thursday, November 03, 2005 5:36 PM
To: cyto-inbox
Subject: PE and PI together?

 

 

Dear Flowers:

 

Can PI be compensated for in a PE-labeled sample that does not exhibit
clearly differentiated positive and negative populations, but a broad peak?
Is measuring cell viability in a separate PI-only sample the norm? 

 

Thanks again for your help!

 

Nancy Ogden, M.Sc
Research Associate
Chemokine Therapeutics Corp.
2386 East Mall, Suite 303
Vancouver, B.C., Canada
V6T 1Z3

 


Tel:  604-822-8848

 

Fax: 604-822-0212

 

 

 

 

 

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