Best blocker?

Mark D'Amico MDAmico at panaceapharma.com
Fri Nov 4 11:03:24 EST 2005


I am using a secondary (goat anti-mouse IgG-PE). I am running the
secondary with the cells (human) alone as one of my controls and get a
bit more binding than I'd like. The incubation takes place in 2% serum (
I have used bovine and human) with similar results. Should I try mouse
serum for the secondary incubations only?
 
 
Mark D'Amico, Ph.D.
Scientist
Panacea Pharmaceuticals
207 Perry Parkway    #2
Gaithersburg, MD   20877
(240) 243-8000 Ext. 206
mdamico at panaceapharma.com
 
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