DNA free cytoplast sorting
g.nebe-von-caron at unipath.com
Fri Nov 4 06:52:38 EST 2005
With bacteria I can sort envelopes that can be distinguished by the
bis-oxonol uptake, reduced light scatter and the absence of DNA staining
by PI and EB. Any reason why you can't use PI?
From: Stephen Sullivan [mailto:sjs93 at hermes.cam.ac.uk] On Behalf Of Dr.
Sent: 02 November 2005 22:19
Subject: DNA free cytoplast sorting
After enucleation treatment, I wish to isolate DNA free ES cell
using FACS. I have two questions:
1. Can FACS be used to isolate cytoplasts? (by using FCS to distingish
cytoplast from whole cells for example.)
2. How can I verify lack of DNA without exposing my cells to UV (cant
I would really appreciate any answers/comments/suggestions. Thanks.
Dr. Stephen Sullivan
The Surani Lab
The Gurdon Institute of Cancer and Developmental Biology University of
Cambridge Tennis Court Road Cambridge CB2 1QR England.
Tel: 01223 334135
More information about the Cytometry