DNA free cytoplast sorting

Dr. Stephen Sullivan Stephen.Sullivan at gurdon.cam.ac.uk
Wed Nov 2 17:19:14 EST 2005


After enucleation treatment, I wish to isolate DNA free ES cell cytoplasts 
using FACS. I have two questions:

1. Can FACS be used to isolate cytoplasts? (by using FCS to distingish the 
cytoplast from whole cells for example.)

2. How can I verify lack of DNA without exposing my cells to UV (cant use 
PI)?

I would really appreciate any answers/comments/suggestions. Thanks.

Stephen 


-- 
Dr. Stephen Sullivan 

The Surani Lab
The Gurdon Institute of Cancer and Developmental Biology
University of Cambridge
Tennis Court Road
Cambridge CB2 1QR
England.
http://www.gurdon.cam.ac.uk

Tel: 01223 334135







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