question about stat3/5 flow

Beverly Barton bartonbe at UMDNJ.EDU
Wed Nov 2 08:51:57 EST 2005


Hi,
Check my papers in PubMed, I have successfully stained for STAT3.

Regards,

Beverly E. Barton, Ph.D.
Assistant Professor
Department of Surgery/Division of Urology
UMDNJ-NJMS  MSB G519
185 S. Orange Avenue
Newark, New Jersey 07103

Telephone			973-972-0662
E-mail				bartonbe at umdnj.edu
Telefacsimile		973-972-3892



On Oct 27, 2005, at 12:45 PM, Kim, YaeJean (NIH/NIAID) wrote:

> Dear All,
>
> I have been trying to stain stat3 (pY705) and stat5 (Y694) by 
> intracellular flow on
> cytokine stimulated eosinophils, but having difficulties to detect the 
> signals.
>
> Is there any special thing that I have to pay attention or be cautious 
> about? I would
> appreciate if somebody can give me advice or protocols on the flow for 
> detecting
> phosphorylated stat proteins?
>
> Thank you very much in advance.
>
> YaeJean
>
>
>
>
>
> YaeJean Kim, M.D.
>
> Laboratory of Parasitic Diseases
> NIH 4/B1-05
> 301-451-1889
> NIAID, National Institutes of Health, DHHS
>
>
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