question about stat3/5 flow

Beverly Barton bartonbe at UMDNJ.EDU
Wed Nov 2 08:51:57 EST 2005

Check my papers in PubMed, I have successfully stained for STAT3.


Beverly E. Barton, Ph.D.
Assistant Professor
Department of Surgery/Division of Urology
185 S. Orange Avenue
Newark, New Jersey 07103

Telephone			973-972-0662
E-mail				bartonbe at
Telefacsimile		973-972-3892

On Oct 27, 2005, at 12:45 PM, Kim, YaeJean (NIH/NIAID) wrote:

> Dear All,
> I have been trying to stain stat3 (pY705) and stat5 (Y694) by 
> intracellular flow on
> cytokine stimulated eosinophils, but having difficulties to detect the 
> signals.
> Is there any special thing that I have to pay attention or be cautious 
> about? I would
> appreciate if somebody can give me advice or protocols on the flow for 
> detecting
> phosphorylated stat proteins?
> Thank you very much in advance.
> YaeJean
> YaeJean Kim, M.D.
> Laboratory of Parasitic Diseases
> NIH 4/B1-05
> 301-451-1889
> NIAID, National Institutes of Health, DHHS
> Disclaimer:
> The information in this e-mail and any of its attachments is 
> confidential and may contain
> sensitive information. It should not be used by anyone who is not the 
> original intended
> recipient. If you have received this e-mail in error please inform the 
> sender and delete
> it from your mailbox or any other storage devices. National Institute 
> of Allergy and
> Infectious Diseases shall not accept liability for any statements made 
> that are sender's
> own and not expressly made on behalf of the NIAID by one of its 
> representatives.

More information about the Cytometry mailing list