pSTAT4 and pSTAT6 stains

Sonia Waiczies sonia.waiczies at charite.de
Tue Jul 12 05:34:33 EST 2005


Dear Flowers
Does anyone have a good method for the specific staining of 
intracellular/nuclear pSTAT4 and pSTAT6 in human PBMC? I am currently using 
the same antibodies for western (where i get a nice signal).... however the 
same antibody (same clone) conjugated with PE gives no signal whatsoever in 
FACS. Would appreciate any help!
Thanks and Best regards
Sonia




Sonia Waiczies PhD
Institute of Neuroimmunology (Director: Prof. Dr. F. Zipp)
www.charite.de/nimmu/
Clinical and Experimental Neuroimmunology
Neuroscience Research Center
NWFZ Geb 2680 R. 04011
Charite Campus Mitte
Schumannstrasse 20/21
10117 Berlin
GERMANY
Phone		+49-30-450539033/51
Fax		+49-30-450539906
Email		sonia.waiczies at charite.de


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