FITC/PE and cellcycle on FACScan
Corver, W.E. (PATH)
W.E.Corver at lumc.nl
Fri Jul 1 09:21:17 EST 2005
I would like to advice DRAQ5. Signal is very well separated from PE.
From: Juergen Ostwald [mailto:juergen.ostwald at med.uni-rostock.de]
Sent: donderdag 30 juni 2005 14:42
Subject: FITC/PE and cellcycle on FACScan
Once more a question to cellcycle and flowcytometry.
I use a FACScan (488nm excitation) will stain lymphocytes with a double marker (FITC/PE)
subpopulations. In this subpopulations I will determine the ploidy (status of cell
cycle). Because of the properties
of PJ I will not use it but don't know whats the best alternative to serve the purpose.
More information about the Cytometry