Ecological question: how to differentiate between species by their pigmentcomposition?

florent crepineau florent.crepineau at laposte.net
Mon Jun 28 10:31:23 EST 2004


I don't know if that will help you but here is a link to a
team doing this kind of thing:

http://www.sb-roscoff.fr/Phyto/cyto.html

Best regards

Florent Crépineau


> Dear all,
> 
>  
> 
> Recently we got an (old) Coulter Epics Elite Flowcytometer.
We try to
> differentiate (marine) photosynthetic organisms by their
> pigmentcomposition and do countings. The organisms, which we
want to
> differentiate are green algae, cyanobacteria and red algae.
We also try
> to count bacteria in seawater samples.  
> 
> As I am quite a novice in flowcytometry,  I am interested in
any good
> tips&tricks to perform this. 
> 
> Although we got a good instruction of a colleague, I have some
> questions, on which I would like to hear your answers:
> 
> -	   What are good discriminators (to put on the axes)?
> 
> -	   Can I analyse fixed samples (with formalin) in a sheath of
> demiwater?
> 
> -	   Is Sybr Gold a good choice to label marine samples? Can
fixed
> samples be put in demiwater?	
> 
> -	   We got a set of different (black) "filters". How can
you see
> which to put in front of the FSC detector?
> 
> -	   Will the kind of beamshaper have substantial influence?
> 
>  
> 
> Thanks in advance for your response,
> 
> With best regards,
> 
>  
> 
> Anita Wijnholds
> 
> NIOO-KNAW 
> 
>  
> 
>  
> 
>  
> 
>  
> 
> 

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