GFP transfection

S Clark S.Clark at sheffield.ac.uk
Fri Jun 25 08:55:08 EST 2004


Hello there

One of my customers is having difficulty with a GFP assay. The GFP is virus
conjugated and following transfection, the cells are all green when looked at
through a microscope, however when looked at on the FACSort they flourescence
is less than that of the untransfected cells.

They have added and anti-GFP antibody which stains positive, so it is obviously
there.

Has anyone got any ideas as to what could be causing this problem, and how we
can go about rectifying it??

Thankyou 

Sue Clark
Flow Cytometry Technician
ICS, DGM
University of Sheffield



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