Brdu PI

Colette Charland Colette.Charland at uvm.edu
Fri Jun 25 10:33:43 EST 2004


Greetings from Vermont,

	 Can anyone explain why it is that Brdu Fitc positive cells 
decrease from 62% to 30% after staining with PI?  Is there some sort of 
quenching or do the cells need to be fixed before the PI step.	 I am happy 
to say that our student has beautiful stimulation and wonderful PI 
staining, but we cannot seem to get reproducible results when we put them 
together.
	 Thanks in advance for your help

Colette Charland
Research Facility Coordinator
University of Vermont
College of Medicine-Flow Cytometry Core Lab
89 Beaumont Ave.
Given C316
Burlington, VT 05405
Telephone 1-802-656-8742




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