Uptake of antigen by dendritic cells

Max Warncke warncke at mm11.UKL.uni-freiburg.de
Thu Jun 24 03:46:15 EST 2004


Hello Flowers,

I am searching for a method to determine the amount of antigen which has been 
uptaken by DCs. We used FITC labeled FAB-Fragments to simulate the uptake of 
protein and could found them inside the DC by confocal microscopy. I am now 
looking for a quantitive method, maybe something which could be measured by 
FACS. 

Thank you

Max
____________________________________

Max Warncke, Dipl. Biol.

University Freiburg Medical Center
Medicin I
AG Veelken

Lab. Nothnagel / 5.OG
Breisacherstr. 117

fon: +49.761.270- 7196
fax: +49.761.270- 7177

____________________________________




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