Whole liver mouse hepatocyte isolation
ccwhite at u.washington.edu
Wed Jun 23 16:20:16 EST 2004
Has anyone isolated heptocytes from whole liver cell populations
(kupffers,endothelial,fibroblasts,neutrophils) using flow sorting?
Also, any protocols on liver perfusions for primary hepatocyte
isolation would be helpful. I am having viability problems and
problems getting them to disassociate with collagenase.
University of Washington
CEEH Analytical Cytology Core
Dept of Environmental and Occupational Health Sciences
4225 Roosevelt Way NE #100
Seattle, WA 98105
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