Whole liver mouse hepatocyte isolation

Collin White ccwhite at u.washington.edu
Wed Jun 23 16:20:16 EST 2004

Has anyone isolated heptocytes from whole liver cell populations 
(kupffers,endothelial,fibroblasts,neutrophils) using flow sorting? 
Also, any protocols on liver perfusions for primary hepatocyte 
isolation would be helpful. I am having viability problems and 
problems getting them to disassociate with collagenase.
Collin White
Research Scientist
University of Washington
CEEH Analytical Cytology Core
Dept of Environmental and Occupational Health Sciences
4225 Roosevelt Way NE #100
Seattle, WA 98105

Ph# 206-616-4982
Fax# 206-685-4696

More information about the Cytometry mailing list