Whole liver mouse hepatocyte isolation

Collin White ccwhite at u.washington.edu
Wed Jun 23 16:20:16 EST 2004


Flowers,
Has anyone isolated heptocytes from whole liver cell populations 
(kupffers,endothelial,fibroblasts,neutrophils) using flow sorting? 
Also, any protocols on liver perfusions for primary hepatocyte 
isolation would be helpful. I am having viability problems and 
problems getting them to disassociate with collagenase.
-- 
Collin White
Research Scientist
University of Washington
CEEH Analytical Cytology Core
Dept of Environmental and Occupational Health Sciences
4225 Roosevelt Way NE #100
Seattle, WA 98105

Ph# 206-616-4982
Fax# 206-685-4696



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