Sorting - LS threshold?

Pál Jáksó pal.jakso at aok.pte.hu
Sun Jun 20 03:59:24 EST 2004


Hello Ray,

Yes it does because  increasing the treshold for FSC means that the 
cytometer won't see the debris but it does not mean that it's not there 
so you may sort the debris into your sample without any control.

Bests,

Pal Jakso

University of Pecs
Faculty of Medicine
Dept. of Pathology
12. Szigeti str.
H-7643 Pecs, HUNGARY

On Jun 17, 2004, at 20:50, Ray Hester wrote:

> One more question about sorting - does it matter whether or not you 
> increase the forward light scatter threshold (to block out dead/debris 
> events) during the sort?  I seem to remember from somewhere that it 
> was best to leave the FS threshold low, i.e., open as opposed to 
> closed, but have forgotten the logic.
>
> Thanks again.
>
> Ray Hester
> rhester at jaguar1.usouthal.edu
>
>






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