Sorting - LS threshold?
hms at shapirolab.com
Fri Jun 18 17:07:13 EST 2004
Ray Hester wrote:
>One more question about sorting - does it matter whether or not you
>increase the forward light scatter threshold (to block out dead/debris
>events) during the sort? I seem to remember from somewhere that it was
>best to leave the FS threshold low, i.e., open as opposed to closed, but
>have forgotten the logic.
If you leave the threshold low, dead cells and debris register as events
and will be excluded from a sort if you have anticoincidence logic. If you
set the threshold high, the dead cells and debris become invisible to the
system and, if they end up in a drop containing a wanted cell, they will be
sorted along with it.
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