CD69 timing

Cohn, Ronald {HTS~Palo Alto} ronald.cohn at ROCHE.COM
Wed Jun 16 09:23:23 EST 2004


Jose,
 
I haven't looked at mitogen activation, but in co-stimulation assays of
human peripheral blood T-cells with anti-CD3 and a number of other
antibodies and integrins I've found fairly high levels of CD69
expression between 18-24 hrs.
 
Ron 

Ronald Cohn 
Research Scientist II 
Imaging and Cytometry 
Lead Discovery Group 
Roche Palo Alto 

email:	ronald.cohn at roche.com 
ph:   (650) 855-6059 
Fax: (650) 354-7554 

	-----Original Message-----
	From: Preffer, Frederic I [mailto:PREFFER at HELIX.MGH.HARVARD.EDU]

	Sent: Monday, June 14, 2004 12:03 PM
	To: Cytometry Mailing List
	Subject: RE: CD69 timing
	
	
	jose
	 
	my recollection was this antigen was upregulated at ~ 4-6 hours
post activation. i dont remember when it down-regulated though,
w/respect to its maintained presence at 24 hours
	 
	F Preffer
	 

		-----Original Message-----
		From: Jose Benito [mailto:jbenito1 at hotmail.com] 
		Sent: Monday, June 14, 2004 10:55 AM
		To: Cytometry Mailing List
		Subject: CD69 timing
		
		
		Hi everyone, I want to study the effect of a certain
drug on CD69 expression after mitogen activation of human blood
lymphocytes. What is the best timing to analuze this marker?. Is 24 hrs
after activation too late?
		Many thanks
		Jose Benito
		Madrid
		Spain

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