CD69 timing

Hodge, Greg (HAEM) hodgeg at mail.wch.sa.gov.au
Tue Jun 15 17:32:39 EST 2004



> -----Original Message-----
> From: Jose Benito [SMTP:jbenito1 at hotmail.com]
> Sent: Tuesday, 15 June 2004 12:25 AM
> To:	Cytometry Mailing List
> Subject:	CD69 timing
> 
> Jose,
> 
> I have found 5 ug/ml PHA in freshly stimulated blood gives around 50%
> positive T cells after 24 hrs- the percentage would be possibly much more
> in isolated lymphocytes.
>  
> Greg
> 
> 
> 
> 
> 
> Hi everyone, I want to study the effect of a certain drug on CD69
> expression after mitogen activation of human blood lymphocytes. What is
> the best timing to analuze this marker?. Is 24 hrs after activation too
> late?
> Many thanks
> Jose Benito
> Madrid
> Spain
> 
>   _____  
> 
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