JC-1

Piotr Wilczek mildes at post.pl
Wed Jun 16 04:32:22 EST 2004


Hello

Is anyone working with JC-1 using a Ortho Diagnostic Instrument. I try to
estimate the mitochondrial potential in a spider cells but the green
fluorescent are very hight. I try  reduce extremaly the green fluorescent
amplfire, to obtain in the control ( no treated fresh lymphocyte) cytogram
only a red fluorescent. It looks good but I'm not sure about the
compensation and the amplifire if the green fluorescent is not to low. Thank
you for any sugestion.

Wilczek Piotr
Biotechnology Laboratory
Heart Prosthesis Institute
Zabzr
POLAND
mildes at post.pl






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