janet dow jldow at unity.ncsu.edu
Tue Jun 15 08:09:49 EST 2004

Dear Margaret:

Unfortunately my experience has been incredibly varied.  I have seen 
antibodies that had been expired for years work wonderfully and have 
seen antibodies that are recently expired work poorly.	I think you 
really have to test the antibodies and have non expired controls to 
compare them too. I also think that lots of times it is the 
flourochrome that has diminished, not the antibody binding.

Sorry I can't be more help.


>Dear All,
>What is the consensus about expired antibodies and flow cytometry -
>and is there a use for those that are expired.
>Any comments would be appreciated.

Janet Dow
Laboratory Research Specialist and Manager
Flow Cytometry and Cell Sorting Facility
North Carolina State College of Veterinary Medicine
Room C-309
Raleigh, NC 27606

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