Effects of freezing and DMSO on expression

Kathy Heel kheel at biaf.uwa.edu.au
Mon Jun 14 02:06:58 EST 2004

Dear FLOWers,

Has anyone experienced CD25/CD69 upregulation after freezing of cells in a
10% DMSO solution? If so, do you know if it is the freezing or the DMSO that
is causing the upregulation?

Experiment note: Lymphocytes were harvested at day 0 from uninfected mice,
washed and frozen at -80C in a solution of 90%FCS & 10%DMSO. Upon defrosting
(after 6-12 months storage), the cells were washed and stained with
anti-CD4, andti-CD25 and anti-CD69 and analysed using a FACSCaliber. Both
CD25 and CD69 were found to be substantially upregulated compared to
previous experiments with unfrozen cells.

Thanks in advance


Dr Kathryn Heel
Biological Imaging and Analysis Facility
The University of Western Australia
Level 1, L Block, QEII Medical Centre
Nedlands WA 6009

Ph +61 8 9346 4525
Fx +61 8 9346 2912
Mb +61 0 416 085 960
kheel at biaf.uwa.edu.au

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