JC-1 and fixation

Ronald Rabin rrabin at helix.nih.gov
Thu Jun 10 09:58:14 EST 2004


no, you cannot fix them.  the dye leaks into all the cells if they are 
fixed.

ron

On Jun 8, 2004, at 1:52 PM, Melendez, Johana wrote:

> Hello !
> I will like to know if JC-1 stained cells can be fix for later 
> acquisition.	I read a lot of literature and could not find anything 
> about this regard.  My guess it's no, but I'm not sure.  If anybody 
> can answer this question for me I'll appreciate it.
> Thanks
> Johana
>
> Johana Melendez, MS
> Research Associate
> Flow Cytometry Laboratory
> H. Lee Moffitt Cancer Center
> 12902 Magnolia Dr.
> Tampa, Florida 33612-9497
> Phone (813) 745-6610
> Lab. phone (813) 972-8400 x. 2005
> Fax (813) 903-7140
>  <<Melendez, Johana.vcf>>
>
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Ronald L. Rabin, M.D.
Senior Staff Fellow
Laboratory of Immunobiochemistry
DBPAP/OVRR
Center for Biologics Evaluation and Research
U.S. Food and Drug Administration
29 Lincoln Drive (MSC-4555)
Building 29, Room 129
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phone:	301.496.8806
fax:	301.402.5177
email:	rr84g at nih.gov

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