Acridine Orange

Joanne Lannigan joannelannigan at virginia.edu
Mon Jun 7 15:20:40 EST 2004


Hi Karen:
After using AO on our FACSCalibur we have used 50% Bleach for 10 minutes
followed by DH2O for 10 minutes and have not had any carryover problems.

Joanne Lannigan, MS
Director, Flow Cytometry Core Facility
University of Virginia
Jordan Hall, Room 7067
P.O. Box 800734
Charlottesville, VA 22908-0734
Office: 434-924-0274
Lab: 434-243-2695
Fax: 434-982-1071
email: joannelannigan at virginia.edu

> -----Original Message-----
> From: Karen Creswell [mailto:creswelk at georgetown.edu]
> Sent: Friday, June 04, 2004 12:17 PM
> To: Cytometry Mailing List
> Subject: Acridine Orange
> 
> Does anyone routinely run acridine orange through their
> FACScan/FACSort?  I would like suggestions for cleaning the instrument
> afterwards.
> Thanks for your help,
> Karen
> 
> --
> Karen Creswell, Ph.D.
> Director, Flow Cytometry/Cell Sorting Shared Resource
> Lombardi Cancer Center
> Georgetown University Medical Center
> Research Bldg W304
> Box 571469
> Washington, DC	20057-1469
> 202-687-2233
> creswelk at georgetown.edu





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