Nonreadable FCS 3 files from Coulter FC 500 cytometer

J. Paul Robinson jpr at flowcyt.cyto.purdue.edu
Fri Jun 4 21:02:48 EST 2004


We notified Coulter of the error in their software a 
long time ago. I dont know if htey are going to fix it.
regards
paul robinson



On 3 Jun 2004 at 16:55, maciej simm wrote:

> We are currently working on adding the support of these files in the Windows
> version of FlowJo, and we notified Coulter of our findings in our efforts so
> far. There is a keyword $PnB which specifies the byte length for the parameter
> "n". In the LMD files in question, this value is seems to be interfering with
> the interpretation of this data by software which requires strict FCS
> compliance. 
> 
> I don't want to start a big debate over standards (ok, how about a medium
> one?) but life would be a lot easier for all FCS analysis software
> manufacturers if the data would be written in a consistent way by all
> manufacturers who develop acquisition software.
> 
> I will post again once we've got a build of FlowJo for Windows that supports
> this format. 
> 
> I'm also looking forward to the utility Paul mentioned in an email earlier
> today. I couldn't find it on CD8, what is it called? I looked in the
> /content/software folder. 
> 
> Maciej Simm
> Tree Star Inc.
> Technical Support
> 
> 
> 
> > -----Original Message-----
> > From: Tomas Kalina [mailto:tomas.kalina at lfmotol.cuni.cz]
> > Sent: Wednesday, June 02, 2004 4:13 AM
> > To: Cytometry Mailing List
> > Subject: Nonreadable FCS 3 files from Coulter FC 500 cytometer
> > 
> > Hello,
> > 
> > we are running a cooperative minimal residual disease study with people
> > using different platforms (BD and Coulter). For data analysis we want to
> > use a centraly designed templates made in Flow Jo software.
> > Unfortunately our colleague's Coulter-FC 500 cytometer is producing FCS
> > 3 files that are not readable by any other software (Flow Jo or
> > CellQuest).
> > 
> > The troubled instrument is Coulter FC 500 with the Cytomics RXP software.
> > 
> > Doeas anybody have a suggestion how to overcome the Coulter FCS 3
> > incompatibility?
> > 
> > Thank you Tomas Kalina
> > 
> > --
> > Tomas Kalina,M.D.
> > Institute of Immunology
> > Charles University - 2nd Faculty of Medicine
> > tomas.kalina at centrum.cz
> > fax (+420) 22443 5962
> > http://www.lf2.cuni.cz/clip/
> > phone (+420) 22443 5968, (+420) 22443 5969, (+420) 22443 2084
> > postal address:	V uvalu 84
> >				150 06 Praha 5
> >				Czech Republic
> > 
> 
> 
> 


J.Paul Robinson, PhD		 PH:(765)4940757
Professor of Immunopharmacology
Professor of Biomedical Engineering  
Purdue University	   FAX:(765)4940517
EMAIL:jpr at flowcyt.cyto.purdue.edu
WEB: http://www.cyto.purdue.edu     

Have you seen our new HCS webpage?
http://www.cyto.purdue.edu/hcs





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