ask the Profis

rafaelnr rafaelnr at
Fri Jun 4 20:41:56 EST 2004

Dear Flowers, I want to pass on a couple of questions that a professor in our 
department forward to me.
How much time is needed to clean the lines of a sorter between samples in 
order to minimize the carry over? 1 minute, 5 minutes, 10 minutes, 30 minutes, 
one hour , two hours, one day?
Is it needed to run alignment beads and realignment the instrument between 
each sample during a multiple samples sort procedure?
How often during a long sort do you check the side streams and the drop delay?
I appreciate very much all your comments and suggestions
Yours Rafael

Dr. Rafael Nunez MD.
Assistant Professor in Medicine-Hematology/Oncology and Pathology 
Director UIC-Cancer Center Flow Cytometry Core Facility
Hematology-Oncology Section, Department of Medicine
Department of Pathology
University of Illinois at Chicago
MBRB, Room 3211/3168, M/C 734
900 South Ashland Ave. 
Chicago, IL 60607

Phone: 312 3551448 and 312 3550123
Fax: 312 4137963
Email: rafaelnr at

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