Nonreadable FCS 3 files from Coulter FC 500 cytometer
Robert C. Leif
rleif at rleif.com
Wed Jun 2 16:03:41 EST 2004
The problem is that FCS is a very loose standard, which was not based on a
requirements document. Present software engineering practices require a
requirements document, which is of great use when one is designing tests to
validate software or even in the creation of a manufacturer's compliance
The use of XML schema as the basis of a standard has the benefit that
validation tools exist for schema. Unfortunately, progress in replacing FCS
with an XML based standard, such as CytometryML, has been slow.
From: Tomas Kalina [mailto:tomas.kalina at lfmotol.cuni.cz]
Sent: Wednesday, June 02, 2004 4:13 AM
Subject: Nonreadable FCS 3 files from Coulter FC 500 cytometer
we are running a cooperative minimal residual disease study with people
using different platforms (BD and Coulter). For data analysis we want to
use a centraly designed templates made in Flow Jo software.
Unfortunately our colleague's Coulter-FC 500 cytometer is producing FCS
3 files that are not readable by any other software (Flow Jo or CellQuest).
The troubled instrument is Coulter FC 500 with the Cytomics RXP software.
Doeas anybody have a suggestion how to overcome the Coulter FCS 3
Thank you Tomas Kalina
Institute of Immunology
Charles University - 2nd Faculty of Medicine
tomas.kalina at centrum.cz
fax (+420) 22443 5962
phone (+420) 22443 5968, (+420) 22443 5969, (+420) 22443 2084
postal address: V uvalu 84
150 06 Praha 5
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