GFP-Cy5 compensation

Li Kim Lee lklee at rci.rutgers.edu
Tue Jun 1 16:22:16 EST 2004


Dear Flowers


I would like to try delivering labelled DNA to GFP-transfected cells. So I
am considering labelling the DNA with perhaps rhodamine or Cy5. Does anyone
have experience and/or opinions on this with respect to the overlapping
spectra, and hence gating and compensation? 


Also, using "red" labels would make it impossible to add in a dye like 7AAD
(to stain for dead cells), right?


Thanks in advance.


Li Kim Lee

Dept of Chem & Biochem Eng

Rutgers University

-------------- next part --------------
HTML attachment scrubbed and removed


More information about the Cytometry mailing list