GFP-Cy5 compensation

Li Kim Lee lklee at
Tue Jun 1 16:22:16 EST 2004

Dear Flowers

I would like to try delivering labelled DNA to GFP-transfected cells. So I
am considering labelling the DNA with perhaps rhodamine or Cy5. Does anyone
have experience and/or opinions on this with respect to the overlapping
spectra, and hence gating and compensation? 

Also, using "red" labels would make it impossible to add in a dye like 7AAD
(to stain for dead cells), right?

Thanks in advance.

Li Kim Lee

Dept of Chem & Biochem Eng

Rutgers University

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