Isolation of granulocytes in Chediak Higashi Syndrome or in Specific Granulocyte deficiency

von Bernuth Horst vonbern at necker.fr
Tue Jun 1 07:07:35 EST 2004


Dear collegues!

Is there anybody who has ever isolated granulocytes from a patient 
with Specific granue deficiency (SGD) or Chediak Higashi Syndrome 
(CHS) ? If so: How did you prepare the cells of the patients with SGD 
and CHS for FACS analysis ? Do you have any experience with Dextran 
Radioselectan gradients or other gradients for the isolation of 
granulocytes in these patients ? Have you performed a lysis of 
erythrocytes prior to FACS analysis ?
I am a little concerned whether the Dextran RAdioselectan gradient 
will work in the usual way in SGD and CHS and whether lysis may harm 
granulocytes of these patients quicker than those of healthy 
controls...
Any word of advice would be strongly appreciated...
Thank you in advance

Horst



Horst von Bernuth, MD
Laboratoire de Génétique Humaine des Maladies Infectieuses
Unité Mixte de Recherche Université René Descartes-INSERM U550

Faculté de Médecine Necker-Enfants Malades
156 rue de Vaugirard
75015 Paris, France, UE

Tel 01 40 61 53 07
Copie 01 40 61 56 88
E-mail vonbern at necker.fr
--------------------------------------------------------------
Horst von Bernuth, MD
Laboratory of Human Genetics of Infectious Diseases
University of Paris René Descartes-INSERM U550

Necker-Enfants Malades Medical School
156 rue de Vaugirard
75015 Paris, France, EU

Voice 33 1 40 61 53 07
Fax 33 1 40 61 56 88
E-mail vonbern at necker.fr




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