hms at shapirolab.com
Thu Jul 1 19:54:53 EST 2004
Josef Schulze-Horsel wrote:
>I'm searching for a possiblity to permeabilize gram positive and/or
>negative bacteria for intracellular staining (e.g. PI).
>Any ideas? Any experiences with digitonin or glutaraldehyde?
The lantibiotic nisin will form an 8 Angstrom pore in bacterial cytoplasmic
membranes, making the orgnaisms permeable to PI, TO-PRO-3, and other
relatively small molecules. The concentration needed varies with the
organism. This strategy won't work if you are trying to get larger
molecules, such as antibodies and nucleic acid probes, into the bacteria,
so you might want to tell us more about the range of intracellular stains
you'd like to use.
More information about the Cytometry