His tag antibody

Peter Nagy pnagy at gwdg.de
Thu Feb 26 14:02:29 EST 2004

Hi Flowers,
We transiently transfect cells with a 6His-tagged protein, and would like 
to stain them with an anti-His antibody in flow cytometry. We plan to use 
formaldehyde-fixed cells. We have checked several anti-His antibodies from 
different companies, but they never really spell out if the antibody works 
on formaldehyde-fixed cells, only on native proteins and methanol-fixed 
cells for immunofluorescence labeling and on denatured epitope for 
westerns. Does anyone have experience with an anti-His tag antibody that 
works on formaldahyde-fixed cells?

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