Luminescent metals

Adrian Rubio arubio at biotechimaging.com
Fri Mar 29 14:36:47 EST 2002


Flow-ers,
what about packaging the unknown metal sample and a secondary/tandem dye in
a liposome?

Laser----> Metal(short life high conc)----->Dye (long life lower conc)----->
Data (MMMMM DATA!)

Adrian

----- Original Message -----
From: "Howard Shapiro" <hms at shapirolab.com>
To: cyto-inbox
Cc: "Cytometry Mailing List" <cytometry at flowcyt.cyto.purdue.edu>
Sent: Thursday, March 28, 2002 3:38 PM
Subject: Re: Luminescent metals


>
> Julie Nelson wrote-
>
> >I just finished talking to a researcher who has some exciting ideas
> >about detecting luminescent metals using flow cytometry, specifically
> >Eu, Tb and Ho.  Has anyone every used flow to analyze these?  Will UV
> >excitation (351 nm) work?
>
> These rare earth metals have been used as immunufluorescent labels in
> chelated form. Their fluorescent lifetimes are hundreds of microseconds,
> making them incompatible with conventional commercial flow cytometers,
> although they have been detected in special laboratory-built instruments.
>
> -Howard
>
>
>




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