Bone marrow filters

Olson, Douglas DOLSON4 at PARTNERS.ORG
Tue Mar 26 13:26:11 EST 2002


Dear Rees:
This is something we do that makes our lives much easier for filtering BM
filters from the clinic (with the ultimate goal of using them for long term
cultures [LTC-IC], where sterility is a huge concern).

We buy filter from a company called Small Parts, Inc. (you can find them on the
web, or customer service # is 1-800-220-4242, they are at 13980 N.W. 58th Court,
Miami Lakes, Florida) - they sell all sorts of filter mesh sizes (20uM, 100uM,
whatever).  You can buy it in any size you want - by the foot, yard, etc. -
theyhave many options - or they will custom cut the mesh if you want a
"not-so-normal" size.
We usually get a few yards worth and then don't have to worry about it for
several months.

We take the filters Bill Telford mentioned that Miltenyi sells and recycle them
- they simply pull apart after use (we soak in bleach to decon then rinse in
water), and we then slip in a piece of mesh of desired size, clip the filters
back together, and autoclave it to get it sterile again.  This is a little more
labor intensive than buying them, but really takes no time at all and is MUCH
cheaper.  Also, it affords the convenience of having the Miltenyi "cup" attached
to the filter whereby it can sit right on top of a 15mL conical tube or standard
flow cytometry tube.

If you did npot go this route, you can still try Small Parts, Inc. for the mesh
filter.

Good luck!

do

-----------------------------------------------------------
Douglas P. Olson
Experimental Hematology/AIDS Research Center
Massachusetts General Hospital
Harvard Medical School
149 13th Street
Boston, MA 02129
(617)724-2668 - Phone
(617)726-4691 - Fax
Dolson4 at partners.org - Email



-----Original Message-----
From: William Telford
To: cyto-inbox
Sent: 3/25/02 4:16 PM
Subject: Re: Bone marrow filters


Hi Rees...

Miltenyi Biotec makes a cup-style preseparation filter for their columns
(#130-041-407) that looks quite similar to the Partec filters - however,
they list the mesh size as 30 um, not 100.  BD also makes cup filters
for
the Medimachine unit (called "filcons") that come in different mesh
sizes
and might be substitutable (100 um sterile are #340637 for 100 count,
US$182).  BD Falcon also makes sterile 40, 70 and 100 um cell filters
that
fit 50 ml tubes (100 um is #352360).  We like to use these because they
are
relatively cheap and sterile - you just have to use them cautiously for
tubes that are less than 50 mls.  BD Falcon also makes their 12 x 75 mm
FACS tubes with the filter cap, 40 um mesh I believe.

As I recall, the Partec filters can be pulled apart, cleaned, their mesh
replaced with Nytex 100 um screening and they can be reused.  I don't
know
if the plastic can be autoclaved, however.

Good luck,

Bill Telford
NCI-NIH

At 05:08 PM 3/25/2002 +1100, Rees Beth wrote:

>Hi Flowers
>
>Help!!!
>
>We've been using Partec's 100um filters to filter out bone particles
and
>cell clumps before staining bone marrow cells.
>
>I'm now told we're the only people in Australia who are using these,
and our
>supplier will not bother to import them after our latest order.  What
are
>other people using, and where can I get it ?
>
>beth
>
>Beth Rees
>Flow Cytometry Lab
>Pathology Dept
>Royal Hobart Hospital
>Hobart, Tasmania
>Australia
>
>ph. 03 6222 8913




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