Counting murine monocytes in blood
simon.watson at ed.ac.uk
Wed Mar 27 06:23:02 EST 2002
Some coworkers in my lab would like to count mouse monocytes in peripheral whole blood using flow cytometry. They tried this using a cytometer (in the local teaching hospital) that was setup for human blood but had no success.
Is it possible to gate for murine moncytes on FSC/SSC or is a fluorochrome-labeled antibody system required?
I presume for absolute and comparative enumeration it would be best to use fluorescent beads as a counting standard. Do you agree?
If anyone could suggest a method or guide us to a reference paper we'd be very grateful.
MRC Centre for Inflammation Research
University of Edinburgh Medical School
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