antibodies into viable cells
rhester at jaguar1.usouthal.edu
Tue Mar 26 12:43:40 EST 2002
The following was sent to me directly so thought I would pass it along.
A colleague passed your question from the cytometry listserve on to me
and I thought I'd reply with a novel method I came across recently.
Feel free to post it. In a recent Nature Biotechnology article (Morris
MC, Depollier J, Mery J, Heitz F, Divita G. A peptide carrier for the
delivery of biologically active proteins into mammalian cells. Nat
Biotechnol. 2001 Dec;19(12):1173-6.) they linked a couple of different
Abs to a Pep1 fragment, a protein transduction domain, and successfully
got them into cells. High efficiency, low toxicity.
C. Mark Lies, Ph.D.
Technical Marketing Scientist
Miltenyi Biotec, Inc.
12740 Earhart Ave.
Auburn, CA 95602
Toll free: (800)367-6227
mlies at miltenyibiotec.com
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