sheath fluid: thanks

Stojan Dimitrov stoianski_fl at yahoo.com
Mon Mar 25 14:10:16 EST 2002


Dear flow-ers,
Thanks a lot for your help. We have checked all
potential sources for contamination and/or impurity in
the fluidic system. Eventually we have resolved the
problem. Our APC beads tube was contaminated/unclear
(instead of using one drop we just pipete 15 mkl with
a tip and we have possibly contaminated the tube) and
now we just are using another lot.
Best regards,
Stojan

Central Laboratory of Immunology, National Center of
Infectious and Parasitic Diseases 1504 Sofia, 26,
Yanko Sakazov Blvd, Bulgaria



--- Stojan Dimitrov <stoianski_fl at yahoo.com> wrote:
>
> Dear flowers,
>
> In our laboratory we have a problem with
> disinfection
> of the fluidic system of our Flow Cytometer. We use
> a
> self made sheath fluid which contains azid as a
> substance to stop the grouth of bacteria.
> Unfortunetly
> when we calibrate the cytometer with “Calibrite
> beads”
> there are many events above the treshold of 300 on
> FSC. Is there any substance which we might add to
> the
> sheath fluid to stop the contamination of the
> fluidic
> system and which would be harmless to the optics and
> silicon tubes.
> Any help will be appreciated. Thank you in advance
>
> Best regards,
> Stoian Dimitrov
>
> Central Laboratory of Immunology, National Center of
> Infectious and Parasitic Diseases 1504 Sofia, 26,
> Yanko Sakazov Blvd, Bulgaria
>
>
>
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