Bone marrow filters

William Telford TelfordW at mail.nih.gov
Mon Mar 25 16:16:51 EST 2002


Hi Rees...

Miltenyi Biotec makes a cup-style preseparation filter for their columns
(#130-041-407) that looks quite similar to the Partec filters - however,
they list the mesh size as 30 um, not 100.  BD also makes cup filters for
the Medimachine unit (called "filcons") that come in different mesh sizes
and might be substitutable (100 um sterile are #340637 for 100 count,
US$182).  BD Falcon also makes sterile 40, 70 and 100 um cell filters that
fit 50 ml tubes (100 um is #352360).  We like to use these because they are
relatively cheap and sterile - you just have to use them cautiously for
tubes that are less than 50 mls.  BD Falcon also makes their 12 x 75 mm
FACS tubes with the filter cap, 40 um mesh I believe.

As I recall, the Partec filters can be pulled apart, cleaned, their mesh
replaced with Nytex 100 um screening and they can be reused.  I don't know
if the plastic can be autoclaved, however.

Good luck,

Bill Telford
NCI-NIH

At 05:08 PM 3/25/2002 +1100, Rees Beth wrote:

>Hi Flowers
>
>Help!!!
>
>We've been using Partec's 100um filters to filter out bone particles and
>cell clumps before staining bone marrow cells.
>
>I'm now told we're the only people in Australia who are using these, and our
>supplier will not bother to import them after our latest order.  What are
>other people using, and where can I get it ?
>
>beth
>
>Beth Rees
>Flow Cytometry Lab
>Pathology Dept
>Royal Hobart Hospital
>Hobart, Tasmania
>Australia
>
>ph. 03 6222 8913





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