Peripheral blood CD 34 counts used in assessing apheresis collect ions

Pat McMahon Pat.McMahon at
Mon Mar 25 18:33:51 EST 2002

Dear Flow folks:

I am relatively new to this users group and new to flow so please bear with

Our facility currently collects CD34+ stem cells for stem cell
transplantation using apheresis.
Our Apheresis collection center is interested in performing peripheral blood
CD34 stem cell enumeration on patients prior to aphersis collection. The
goal is to determine if a patient has an adequate number of CD34+ stem cells
before performing the apheresis collection. They do not wish, necessarily,
to collect the entire CD34 dose in one collection but, rather to ensure that
there is adequate cd34+ stem cells such that 2-3 collections would yield an
adequate dose for transplantation.

Does anyone have any experience with this type of data. Are there some
guidelines as to how many cd34+ stem cells /ul of peripheral blood are
necessary to give an acceptable apheresis collection dosage?

I am using an Epics XL and the Beckman/Coulter Stem-kit for CD34

Thanks for any help.

Patrick McMahon
Avera Mckennan Hospital
Sioux Falls, SD
email  Pat.McMahon at
Fax: 605-322-7150

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