a question on cell cycle analysis with PI

Yalin Guo guo at mm11.ukl.uni-freiburg.de
Tue Mar 26 09:12:44 EST 2002

I am doing a cell cycle analysis using cultured and non-cultured
human CD34+ cells. I got a protocol with PI staining (0.1% Na
Citrate, 0.1% Triton X-100, 20 ug/ml PI), but without fixation
and RNase. I should be very grateful to get any suggestions for
this method.


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