Missing Myeloma cells

Melvin Hasseler hasseler at hsc.usc.edu
Fri Mar 22 13:47:22 EST 2002

It has been our experience that when we add CD45 PerCP as a third color to our cytoplasmic Kappa/138 & Lambda/138 tubes on bone marrow samples suspected of having myeloma cells, the cells seem to have variable amounts of CD45 or slam against the left with zero CD45 PerCP staining. In our two color analyzes myeloma cells are negative to dim for CD45. Our compensation appears to be correct for the other markers, bright or dim, when we add CD45 PerCP.   

Mel Hasseler
Supervisor, USC Flow Cytometry Section In Los Angeles
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