Basophills

Calman Prussin CPRUSSIN at niaid.nih.gov
Thu Mar 21 17:36:14 EST 2002


There are no basophil specific surface markers. There are 2 basophil granule
specific markers, BB1 and 2D7, which can be used with I/C staining. .
Because of the small numbers of basophils (about 1%) we have generally use 2
markers to identify them. We stain with anti-IgE and a panel of lineage
markers (CD2, 14, 16, 19). IgE+, lin- cells are >99% basophils. MHC II
positive dendritic cell precursors make up the balance.

Calman
> ----------
> From:		Efros, Lyubov
> Sent:		Wednesday, March 20, 2002 17:10
> To:	Cytometry Mailing List
> Subject:	Basophills
>
>
>
>      Dear Flow-ers,
>
>	I am looking  if someone have experience using different cell
> surface markers for  basophils from Human peripheral whole blood  (
> without
> preparing PBMC). Also,	if it possible to do enrichment  of
> basophils,
> without separating mononuclear cells first.
>
>	    Any information is greatly appreciated.
>
>     Lyuba Efros
>     Preclinical Department
>    PDL, Fremont, CA
>     lefros at pdl.com
>
>
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