CPRUSSIN at niaid.nih.gov
Thu Mar 21 17:36:14 EST 2002
There are no basophil specific surface markers. There are 2 basophil granule
specific markers, BB1 and 2D7, which can be used with I/C staining. .
Because of the small numbers of basophils (about 1%) we have generally use 2
markers to identify them. We stain with anti-IgE and a panel of lineage
markers (CD2, 14, 16, 19). IgE+, lin- cells are >99% basophils. MHC II
positive dendritic cell precursors make up the balance.
> From: Efros, Lyubov
> Sent: Wednesday, March 20, 2002 17:10
> To: Cytometry Mailing List
> Subject: Basophills
> Dear Flow-ers,
> I am looking if someone have experience using different cell
> surface markers for basophils from Human peripheral whole blood (
> preparing PBMC). Also, if it possible to do enrichment of
> without separating mononuclear cells first.
> Any information is greatly appreciated.
> Lyuba Efros
> Preclinical Department
> PDL, Fremont, CA
> lefros at pdl.com
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