getting antibodies into viable cells

Gerhard Nebe-von-Caron Gerhard.Nebe-von-Caron at Unilever.com
Thu Mar 21 19:24:14 EST 2002


I would guess that TAT peptides should be the perfect way to achieve that. They
are derived from an ancient bacterial transport system through the cell
membrane and can be coupled or in this time of molecular techniques simply
fused to your molecule of interest. However I think there might already be some
patents on that.

Regards

Gerhard Nebe-von-Caron

Research Scientist
Applied Science & Technology Group
SEAC - Safety and Environmental Assurance Centre
Unilever Research, Colworth Laboratory, Sharnbrook, UK - MK44 1LQ

Tel: +44 (0)1234 264822, Fax: +44 (0)1234 222552
E- mailto:Gerhard.Nebe-von-Caron at unilever.com



-----Original Message-----
From:	ray hester [SMTP:rhester at JAGUAR1.USOUTHAL.EDU]
Sent:	Thursday, March 21, 2002 2:20 PM
To:	Cytometry Mailing List
Subject:	getting antibodies into viable cells


Greetings,

A recent discussion on the Confocal Listserv, concerning DAPI staining of
viable cells, prompts me to send the following from a colleague:

Is there a way to deliver antibodies, or their fragments, into viable cells?
We have heard of using liposomes, but wondered if perhaps newer techniques
have emerged.

Thanks.

Ray Hester
Univ. of South Alabama
Mobile, AL 36688

rhester at jaguar1.usouthal.edu




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