FITC vs Alexa 488
LBloom at Phylos.com
Fri Mar 22 09:08:27 EST 2002
The difference is very clear in fluorescence microscopy: FITC bleaches
significantly faster than Alexa 488. We haven't compared the same antibody
conjugated both ways, but among commercially-available secondary reagents,
Alexa 488 conjugates seem a bit brighter in the flow cytometer too.
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> From: John Krowka
> Sent: Thursday, March 21, 2002 11:58 AM
> To: Cytometry Mailing List
> Subject: FITC vs Alexa 488
> On Molecular Probes' website are claims that Alexa 488 is 6-7 times
> than FITC. Preliminary evidence from our conjugations of the same antibody
> with FITC or Alexa 488 indicate no advantage from using Alexa 488. Can
> anyone who compared Alexa 488 and FITC share their findings?
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